Journal of Food, Agriculture and Environment

Vol 11, Issue 1,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Molecular characterization and genetic diversity analysis of mandarin genotypes by SSR and SRAP markers


Yildiz Kacar 1*, Aydin Uzun 2, Ilknur Polat 3, Turgut Yesiloglu 1, Bilge Yilmaz 1, Osman Gulsen 2, Onder Tuzcu 1, Müge Kamiloglu 4, Senay Kurt 3,  Ubeyit Seday 5

Recieved Date: 2012-11-20, Accepted Date: 2013-01-30


The aim of the present study was to characterize the mandarin germplasm conserved at the Tuzcu Citrus Variety Collection at Cukurova University in Turkey using simple sequence repeat (SSR) and sequence-related amplified polymorphism (SRAP) based molecular approaches. Genetic characterization of 65 mandarins was performed using 14 SSR markers and 21 SRAP markers. We originally tested 26 SSR markers, and 14 of these markers were selected due to their high polymorphism information content in the molecular assays. Thirty-eight alleles were detected at 14 loci. The number of alleles per SSR locus ranged from 2 to 4, with a mean of 2.7 alleles. The most informative loci were CAGG 9, CAT 01 and TAA 52 (4 alleles/primer). From the SRAP analysis, a total of 187 alleles were generated, and the polymorphism rate was 77%. The number of alleles detected by a single primer set ranged from 4 to 15, with an average of 8.90. The UPGMA dendrogram, defined by SSR and SRAP markers, revealed the genetic relatedness of the mandarin genotypes. These findings can be used to guide future breeding studies and germplasm management of these mandarin genotypes.


Genetic diversity, mandarin, markers, SSR, SRAP

Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 1
Category: Agriculture
Pages: 516-521

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :