Journal of Food, Agriculture and Environment

Identification and targeting of the pig Rosa26 locus

Author(s):

Huiming Ju ^{1, 2,¶}, Yuefei Yang ^1¶, Lijing Bai ², Lei Huang ², Xin Jiang ¹, Yulian Mu ^{2 *}, Kui Li ^{2 *}

Recieved Date: 2015-01-02, Accepted Date: 2015-03-30

Abstract:

The Rosa26 locus in mice is an ubiquitously expressed gene that has a high homologous recombination efficiency. We have investigated the orthologous gene in pig, Sus scrofa. The mouse-transcribed Rosa26 (mRosa26) sequence was used to screen the pig genome database to identify homologous sequences. Four different pig Rosa26 (pRosa26) promoter sequences were cloned into a luciferase expression reporter vector and a 409-bp sequence was shown to be a core promoter. Zinc finger nucleases were designed based on the pRosa26 exon 1 sequence and used to mediate gene targeting in the pig PK15 cell line. Targeted cell lines were identified and pRosa26 gene targeting verified. β-Galactosidase staining of targeted clones indicated that the Rosa26 promoter was functional. Cell cycle and apoptosis indices demonstrated no significant differences between targeted and control cells. This study confirmed the existence of pig Rosa26 and identified it as a suitable locus for transgenic manipulation.

Keywords:

Pig, Rosa26, ZFNs, friendly loci

---

Journal: Journal of Food, Agriculture and Environment
Year: 2015
Volume: 13
Issue: 2
Category: Agriculture
Pages: 149-155

---

Full text for Subscribers

---

Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

---

Purchase this Article:   20 Purchase PDF Order Reprints for 15

---

Share this article :