Journal of Food, Agriculture and Environment

Vol 1, Issue 3&4,2003
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Lipase-catalyzed synthesis of isoamyl acetate in free-solvent media — optimization using a central composite design


Gabriela A. Macedo *, Gláucia M. Pastore, Maria I. Rodrigues

Recieved Date: 2002-10-23, Accepted Date: 2003-05-16


The catalytic action of two non commercial lipases (E.C. in the synthesis of isoamyl acetate in a solvent-free system, were tested using an experimental design in order to better understand the relationship between the reaction variables. Yields ranging from 67 to 77 % molar conversion were achieved. The synthesis of isoamyl acetate catalyzed by the chosen lipase (Rhizopus sp.) was optimized, in order to maximize the molar conversion (yield, Y) in a solvent-free system. Response surface methodology involving the substrate molar ratio (RM) and amount of lipase (L) was used, and a second order model was employed to generate response surfaces. The optimum conditions to obtain Y max = 80% were: RM= (2:1); L= 8.7% ( w/w), 40°C, and 48 hours of reaction time.


Flavour esters, esterification, response surface methodology, lipase

Journal: Journal of Food, Agriculture and Environment
Year: 2003
Volume: 1
Issue: 3&4
Category: Food and Health
Pages: 59-63

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :