Journal of Food, Agriculture and Environment

Interactions between phytase and different dietary minerals in in vitro systems

Author(s):

Marjina Akter ^1*, Hadden Graham ², Paul Ade Iji ¹

Recieved Date: 2015-05-08, Accepted Date: 2015-09-19

Abstract:

An in vitro experiment was conducted to evaluate the effect of different dietary minerals on phytate hydrolysis by microbial phytase enzyme and to measure the residual phytate content after phytate digestion. Calcium (0, 6, 8, 10 g kg-¹ of diet), Fe (0, 70, 80, 90 mg kg^-1 of diet), Zn (0, 30, 40, 50 mg kg^-1 of diet) or Na (0, 1.5, 2.5 and 3.5 g kg^-1 of diet) were incubated with a Na-phytate solution, with phytase enzyme (500 FTU kg^-1 of diet) at pH 2.5 or 6.5. The phytate concentration was set at 0.27% to mimic the phytate level in a typical corn-soybean meal-based poultry diet. Inclusion of Ca, Zn and Fe significantly (p<0.05) reduced the phytate phosphorus hydrolysis at both pH values but the effect was strongest at pH 6.5. Irrespective of the pH, adding Na also inhibited (p<0.05) phytate phosphorus hydrolysis; however, the negative effect was more pronounced at pH 2.5 than 6.5. Residual phytate content (mg) after phytate digestion was higher (p<0.05) at pH 6.5 than at 2.5 in the presence of Ca, Zn and Fe. The reverse was the case in the presence of Na. These in vitro results demonstrated that increased concentrations of dietary Ca, Fe, Zn and Na could reduce phytate phosphorus hydrolysis, but such a response would need to be tested in animal feeding trials.

Keywords:

In vitro, phytate, phytase, minerals, pH

---

Journal: Journal of Food, Agriculture and Environment
Year: 2015
Volume: 13
Issue: 3&4
Category: Agriculture
Pages: 38-44

---

Full text for Subscribers

---

Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

---

Purchase this Article:   20 Purchase PDF Order Reprints for 15

---

Share this article :