Journal of Food, Agriculture and Environment
Vol 11,
Issue 1,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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Evaluation of genetic diversity and differentiation of Cuscuta campestris (field dodder) ecotypes using ISSR markers
Author(s):
Sara Tajdoost 1*,
Ramazan Ali Khavari-Nejad 1, Fariba Meighani 2, Eskandar Zand 2, Zahra Noormohammadi 1Recieved Date: 2012-09-21, Accepted Date: 2013-01-28
Abstract:Field dodder (Cuscuta campestris) is an obligate parasite that causes devastating losses in many economically important crops. In the present study, the pattern of genetic diversity within and among C. campestris ecotypes collected from different regions of Iran was assessed by using inter-simple sequence repeat (ISSR) markers. Eight ISSR primers generated a total of 280 bands, of which 269 bands were polymorphic. Polymorphism information content (PIC) and resolving power (RP) averaged 0.6 and 2.9, respectively. UPGMA cluster analysis based on Dice similarity matrix, greatly showed no relationship between genetic divergence and geographical origins of ecotypes. The cluster analysis results and principal component analysis (PCA) plots of the marker were highly concordant. The analysis of molecular variance (AMOVA) showed high genetic diversity (81%) among populations. High genetic differentiation among populations (PhiPT = 0.814, P = 0.01) could be due to high self fertilization and limited gene flow. Understanding genetic variability of the weed can be effective in advancing of management and control strategies to reduce impact of the weed in agricultural fields.
Keywords:Genetic diversity,
inter-simple sequence repeat markers, polymorphism information content, resolving power, Cuscuta campestrisJournal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 1
Category: Environment
Pages: 1072-1075
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