Journal of Food, Agriculture and Environment




Vol 1, Issue 3&4,2003
Online ISSN: 1459-0263
Print ISSN: 1459-0255


Protoplast isolation, culture and application to genetic improvement of woody plants


Author(s):

Jihong Liu *, Xiaoyong Xu, Xiuxin Deng

Recieved Date: 2003-01-19, Accepted Date: 2003-08-13

Abstract:

Traditional breeding of most woody plants is more or less confronted with certain barriers. Protoplast isolation and culture pave way for genetic improvement of woody plants. Since the first try on protoplast isolation and culture was conducted much progress has been made in woody plants, covering most of the important species including fruit trees, such as citrus, apple, pear, mango, kiwifruit, litchi, and forestry trees, such as sandalwood, poplar, eucalyptus, mulberry, conifer, etc. The present paper reviews the general protocols concerning protoplast isolation and culture, summarizes the factors affecting protoplast isolation and culture, introduces the variations, inclusive of chromosome number, morphology and resistance, occurring in protoplast-derived plants. In addition, application of protoplast to genetic improvement and basic study of woody plants are discussed herein, such as cryopreservation, somatic hybridization, genetic transformation and other basic researches.

Keywords:

Protoplast isolation, protoplast culture, woody plants, genetic improvement


Journal: Journal of Food, Agriculture and Environment
Year: 2003
Volume: 1
Issue: 3&4
Category: Agriculture
Pages: 112-120


Full text for Subscribers
Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.


Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :