Journal of Food, Agriculture and Environment

Vol 1, Issue 3&4,2003
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Protoplast isolation, culture and application to genetic improvement of woody plants


Jihong Liu *, Xiaoyong Xu, Xiuxin Deng

Recieved Date: 2003-01-19, Accepted Date: 2003-08-13


Traditional breeding of most woody plants is more or less confronted with certain barriers. Protoplast isolation and culture pave way for genetic improvement of woody plants. Since the first try on protoplast isolation and culture was conducted much progress has been made in woody plants, covering most of the important species including fruit trees, such as citrus, apple, pear, mango, kiwifruit, litchi, and forestry trees, such as sandalwood, poplar, eucalyptus, mulberry, conifer, etc. The present paper reviews the general protocols concerning protoplast isolation and culture, summarizes the factors affecting protoplast isolation and culture, introduces the variations, inclusive of chromosome number, morphology and resistance, occurring in protoplast-derived plants. In addition, application of protoplast to genetic improvement and basic study of woody plants are discussed herein, such as cryopreservation, somatic hybridization, genetic transformation and other basic researches.


Protoplast isolation, protoplast culture, woody plants, genetic improvement

Journal: Journal of Food, Agriculture and Environment
Year: 2003
Volume: 1
Issue: 3&4
Category: Agriculture
Pages: 112-120

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