Journal of Food, Agriculture and Environment




Vol 12, Issue 1,2014
Online ISSN: 1459-0263
Print ISSN: 1459-0255


Molecular detection of Salmonella enterica serovars Typhimurium and Enteritidis in diarrheic calves


Author(s):

Ismail Al Hazmi Mohamed 1, Ashgan Mohamed Hessain 2, Abdullah Abdulaziz Al-Arfaj 3, Ihab Mohamed Moussa 3*

Recieved Date: 2013-10-16, Accepted Date: 2013-01-08

Abstract:

The objective of the present study was to evaluate the Multiplex-PCR for the rapid detection of Salmonella Typhimurium and Salmonella Enteritidis in fecal samples collected from diarrheic and apparently healthy contact calves from farms located in Egypt. Fecal samples were collected from 150 diarrheic calves and 95 apparently healthy contact calves during summer 2012. All samples were examined for the presence of Salmonella species using bacteriological examination and Multiplex-PCR. Salmonella was isolated from 54 (36%) of diarrheic calves and from 29 (30.53%) of apparently healthy contact calves. Salmonella Typhimurium was isolated from 14.67% of diarrheic and 15.79% of contact calves; whereas Salmonella Enteritidis was isolated from 5.33% and 4.21%, respectively. Salmonella Dublin was isolated from 10% of the diarrheic and 8.42% of contact calves but Salmonella Anatum was isolated from 6% of the diarrheic and 2.11% of contact calves. The Multiplex-PCR could detect all the bacteriologically positive samples, in addition, it could detect 11 samples revealed negative results with bacteriological examination.

Keywords:

Salmonella, Multiplex-PCR, diarrheic calves, Salmonella Typhimurium, Salmonella Enteritidis


Journal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 1
Category: Agriculture
Pages: 192-194


Full text for Subscribers
Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.


Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :