Journal of Food, Agriculture and Environment

Fed-batch process development for high cell density cultivation of Lactobacillus fermentum L1

Author(s):

Yu Li, Wen-hang Wang, Meng-Cheng Yao, Jiang Li, Xiao-Guang Liu, Fu-Ping Lu*

Recieved Date: 2013-02-22, Accepted Date: 2013-04-18

Abstract:

To obtain a high cell density of Lactobacillus fermentum L1, fermentation processes were studied to reduce the effect of acid, oxygen and starvation. Both batch and fed-batch fermentations were investigated. For batch fermentation, the pH was determined at 7.0 at the beginning and kept constant at 6.0 when it decreased to 6.0. The optimal agitation speed and aeration were 100 rpm and 0 vvm (air volume/culture volume/min) respectively. For fed-batch fermentation, glucose and nitrogen mixture of all the medium components were identified as the limiting factors for cell growth. The optimized ratio of glucose to nitrogen mixture as a feed medium was 1:1 (v/v). With the feed medium, constant feeding and glucose feedback feeding strategies were compared and the latter was proved to be more effective for cell growth. Finally, glucose feedback feeding strategy was selected as the most effective fed-batch process, with which, the maximal cell density obtained at 36 h was 8.23×10⁹ CFU/mL, which was 1.87 times as much as that of constant feeding and 4.78 times as much as that of batch cultivation.

Keywords:

Lactobacillus fermentum, high cell density cultivation, limiting factor, batch fermentation, fed-batch fermentation

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 2
Category: Food and Health
Pages: 43-47

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