Journal of Food, Agriculture and Environment

Vol 11, Issue 2,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Genetic diversity of coffee (Coffea arabica L.) in Yemen via SRAP, TRAP and SSR markers 


Taha Makerd Al-Murish 1, Adel Ahmed Elshafei 2, 3, Abdullah  Abdulaziz Al-Doss 2, Mohamed Najeb Barakat 2, 4*

Recieved Date: 2013-01-24, Accepted Date: 2013-04-29


This study was conducted to evaluate the efficiency of SRAP, TRAP and SSR primers in detecting genetic variation among 17 C. arabica genotypes collected from the different valleys of Yafea City, Yemen, and estimate genetic similarity coefficients among these genotypes and classify them according to genetic relationships. Results of the present study demonstrated the presence of genetic variation among coffee genotypes within and between valleys. Cluster analysis using SRAP, TRAP, SSR and combined data, grouped the 17 coffee genotypes into three main clusters with Jaccard’s similarity coefficient ranging from 0.81 to 0.98, from 0.90 to 0.99, from 0.69 to 0.90 and from 0.84 to 0.96, respectively. The combined analysis produced similar dendrogram to that produced using SRAP marker analysis. Sixteen SSR primers were used to investigate the discriminative power of each SSR primer by calculating polymorphic information contents. A total of 37 alleles were amplified among 17 coffee genotypes using sixteen SSR primers. The number of amplified alleles per primer varied from one allele as for AJ308738 to seven alleles for AJ250256, with an average value of 2.31 alleles. The polymorphic information content per primer ranged from 0.000 to 0.713 with an average of 0.319. Observed heterozygosity was calculated for each primer and ranged from 0.00 to 1.00 with an average of 0.43. 


Coffea arabica L., genetic diversity, SRAP markers, TRAP markers, SSR markers

Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 2
Category: Agriculture
Pages: 411-416

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :