Journal of Food, Agriculture and Environment

Effect of temperature and light impact on expression of lycopene cyclase gene in tobacco (Nicotiana tabacum L.) 

Author(s):

Yun-Peng Fu ¹, Feng Jia ², Wei-Qun Liu ³*, Zhengyang Zhang ⁴, Tai-bo Liang ⁵

Recieved Date: 2013-01-20, Accepted Date: 2013-04-28

Abstract:

To demonstrate effect of temperature or light intensities on expression of lycopene cyclase gene (lyc-β ), which is one of the critical enzymes in the process of carotenoid synthesis, tobacco leaf discs were treated with different temperatures and light intensities. In this study, orthogonal experiment L₉(3⁴) was adopted to investigate the effect of temperature and light intensity on expression of lyc-β with semi-quantitative RT-PCR. At same time, lyc-β expression and contents of carotenoids in different ecological areas were detected. The results indicated that light was a key factor affecting expression of lyc-β , followed by temperature and the least was the interactions of temperature and light. Results of Duncan’s test showed that three factors had no significance in three levels (P<0.5). Results of different light treatments suggested that expression of lyc-β increased during 2 to 6 h, however, it would inhibit when treatments of light was over 8 h. Moreover, expression of lyc-β and content of carotenoids were significantly different in Sichuan and Henan (P<0.01), and they were about the same level at the same areas. 

Keywords:

Tobacco, lycopene cyclase gene (lyc-β ), orthogonal experiment, temperature, light intensities

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 2
Category: Agriculture
Pages: 449-452

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