Journal of Food, Agriculture and Environment

Bioinformatic and antiviral functional analysis of WW domain containing E3 ubiquitin protein ligase 2 gene

Author(s):

Zhenghu Chen ^1†, Xiaoyan Cui ^1†, Hongwei Yan ^2†, Guanyu Jiang ¹, Yanfei Li ², Rui Wan ¹, Da Wo ², Jianhua Yang ¹, Weidong Zhu ², Shangfeng Liu ^{1 *}

Recieved Date: 2014-02-03, Accepted Date: 2014-04-04

Abstract:

Based on computational analysis, we identified full length cDNA of 14 WW domain containing E3 ubiquitin protein ligase 2 gene (WWP2) (GenBank Accession No. JN712744-57) from 14 different vertebrates ranging from zebrafish, chicken to chimpanzee and human by using human or mouse WWP2 as electronic probe. Previously reported WWP2 sequences from human, mouse and rat were modified by extended their 5’ flank sequence, which is considered to be the transcription start site. All gene sequences are novel and first submitted into GenBank. In this study, we show that the similarity ratio of WWP2 protein sequence range from 73.2% to 99.8% among different vertebrates ranging from zebrafish, chicken to chimpanzee and human. Most of WWP2 genes across different species are highly conserved and have 24 or 25 exons, 870 aa and a typical CpG island within the promoter region. Our analyses indicate that WWP2 sequence has high conservation in vertebrates and may play an important role in cell process. Through further research, we found that overexpression of WWP2 impaired TBK1 and Sendai virus-mediated activation of IFN-stimulated response element (ISRE), IFN-promoter indicating WWP2 is associated with antiviral signaling pathway.

Keywords:

WWP2, computational analysis, gene structure, antiviral response

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Journal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 2
Category: Food and Health
Pages: 118-123

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