Journal of Food, Agriculture and Environment

Random amplified polymorphic DNA-polymerase chain reaction for molecular typing of methicillin-resistant Staphylococcus aureus

Author(s):

Abdullah Abdulaziz Al-Arfaj ¹, Ashgan Mohamed Hessain ^{2 *}, Saleh Abdullah Kabli ³, Hassan Abdullah Hemeg ⁴, Ihab Mohamed Moussa ¹

Recieved Date: 2014-02-06, Accepted Date: 2014-04-09

Abstract:

Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) as an epidemiological marker were used in this study for molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) recovered from Cairo, Egypt. The strains were primary examined by multiplex polymerase chain reaction (multiplex-PCR) for direct detection of S. aureus 16S rRNA, Panton-Valentine Leucocidin (PVL) and staphylococcal cassette chromosome mec (SCCmec) type IVa genes. A total of 105 S. aureus strains collected during the period of 2009 and 2011 from major hospital laboratories and public health centers, Cairo, Egypt, were tested by conventional methods and (multiplex-PCR). Moreover, 45 strains were selected and examined by RAPD-PCR. PCR could detect all 105 bacteriologically identified strains as S. aureus (100%) and could detect the mecA gene in all strains phenotypically resistant to methicillin (100%) at the same time. The mecA gene was detected in 5 strains (4.76%) phenotypically sensitive to methicillin. Only 15 strains (14.28%) recovered from skin and soft tissue infections were positive for PVL and (SCCmec) type IV. RAPD-PCR revealed 4 clusters on the basis of epidemiological data and phylogenetic tree.

Keywords:

Staphylococcus aureus, RAPD-PCR, finger printing, (SCCmec) MRSA, PVL gene

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Journal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 2
Category: Food and Health
Pages: 229-231

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