Journal of Food, Agriculture and Environment

Molecular characterization of glyceraldehyde-3-phosphate dehydrogenase (gapdh) gene from Aspergillus fumigatus

Author(s):

Tarek A. A. Moussa ^{1, 2}*, Dalia M. Ali ², Neveen M. Khalil ², Fatma A. Mostafa ²

Recieved Date: 2013-06-06, Accepted Date: 2013-10-20

Abstract:

First-strand cDNA was reverse transcribed from mRNA of Aspergillus fumigatus mycelium culture. The nucleotide sequence of gapdh was found to contain an ORF of 1083 bp, capable of coding for a protein of 360 amino acid residues. The signal peptide was predicted to be 19 amino acids in length. The alignment of sequence analysis of this fragment with the previously determined nucleotide sequence led to the definition of the gene (gapdh – cDNA, accession no. AB683056). Sequence analysis revealed the presence of a potential site for substrate binding (ASCTTNCV) at position 172-179. Amino acids potentially associated with catalysis were found at amino acid positions 174 (C) and 201 (H). Potential phosphorylation sites were located at positions 13-21, 204-212, 219-227, 272-280 and 339-346. The amino acid residues at positions 7 (D) and 311 (N) corresponded to the putative NAD⁺ binding sites. Amino acids at positions 145 (S), 150 (T), 204 (T) and 205 (R) were found to be probable sites for inorganic phosphate binding. Positions 180 and 222 were found to be amino acid residues that putatively related to the binding of the phosphate from the substrate (T and R). The calculated molecular weight of deduced polypeptide is 38.7 kDa, and the estimated isoelectric point (pI) is 7.28. The deduced amino acid sequence revealed the most abundant amino acid was alanine followed by leucine, whereas the rare amino acids are methionine followed by tryptophan.

Keywords:

Aspergillus fumigatus, glyceraldehyde-3-phosphate dehydrogenase gene, signal peptide, putative sites

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Food and Health
Pages: 235-241

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