Journal of Food, Agriculture and Environment
Vol 11,
Issue 3&4,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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A novel alkalitolerant Bacillus strain produces cyclodextrin glucanotransferase with high efficiency in transglycosylation of rutin
Author(s):
Tao Sun 1,
Bo Jiang 2, Beilei Pan 3 *, Rebaone Letsididi 4Recieved Date: 2013-07-04, Accepted Date: 2013-10-28
Abstract:Cyclodextrin glucanotransferase (CGTase, EC 2. 4. 1. 19) had been used to transglycosylate rutin to increase its solubility and stability in water for food, cosmetic and pharmaceutical industry applications. However, this application was restricted by the availability of a CGTase-producing microbial strain, which has high efficiency in transglycosylation of rutin. Here, we reported a novel microbial strain SK13.002 producing CGTase with high efficiency in rutin transglycosylation. Based on cell morphological, biochem-physiological characteristics, this strain was identified as Bacillus sp. SK13.002. Phylogenetic analysis of 16S rDNA showed that SK13.002 16S rDNA shared 98.5% identity with 16S rDNA of Bacillus amyloliquefaciens NBRC 15535 and 97.5% identity with that of Bacillus vallismortis DSM11031. The CGTase produced by Bacillus sp. SK13.002 transglycosylated rutin more efficiently compared to CGTases isolated from other strains reported. Thus, Bacillussp. SK13.002 could be a potential CGTase source for the transglycosylation of rutin and other food functional components.
Keywords:Bacillus,
cyclodextrin glucanotransferase, rutin, transglycosylationJournal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Food and Health
Pages: 354-358
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