Journal of Food, Agriculture and Environment

Vol 11, Issue 3&4,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Improvement of dihydroxyacetone production by Gluconobater oxydans NH-10 using a two-stage agitation speed operation strategy


Qinghong You 1, 2, 3, 4, Hong Xu 1*, Xiulian Yin 2, 3, 4, Hongyang Zhu 1, Xiaoyan Dai 1

Recieved Date: 2013-05-08, Accepted Date: 2013-10-20


Batch fermentation production of dihydroxyacetone (DHA) by Gluconobater oxydans NH-10 was studied by oxygen supply method using different agitation speed. On the basis of analyzing the three kinetic parameters, namely specific cell growth rate (µ), specific glycerol consumption rate (qs), and specific DHA production rate (qp), a two-stage agitation speed operation strategy was applied to obtain high DHA concentration and productivity. The agitation speed was firstly controlled at 800 rpm to acquire high specific cell growth rate (µ) in 7.5 L fermenter during the first 21 h. Secondly, the agitation was reduced to 600 rpm to keep high qp for DHA accumulation. Finally, the maximum DHA concentration achieved was 139.20±6.15 g L-1 with the productivity of 2.72±0.12 g L-1h-1, the average concentration and productivity of DHA were 10.3% and 10.1% higher than the best results controlled by constant agitation speeds, respectively. Glycerol conversion rate was increased from 79±3.69% to 87.9±3.88%.


Dihydroxyacetone, Gluconobater oxydans NH-10, kinetic parameters, two-stage agitation speed control strategy

Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Food and Health
Pages: 389-393

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