Journal of Food, Agriculture and Environment

Vol 11, Issue 3&4,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Novel enzymatic extraction and purification method for cyclic adenosine monophosphate (cAMP) from Ziziphus jujuba


Chunxia Wang 1, 3, 4, Xueqin Gao 4, Haikuan Wang 1, 3, 4, Yihan Liu 1, 2, 4, Lianxiang Du 4, Hongbin Wang 1, 3, 4, Fuping Lu 1,2,4

Recieved Date: 2013-06-29, Accepted Date: 2013-10-20


Cyclic adenosine monophosphate (cAMP) is an important component in the regulation of intracellular metabolism and biological function. The cAMP content of jujube (Ziziphus jujuba Mill, Chinese date) is particularly abundant and stable, more than any of the other plants researched so far. In this paper we report the development of a novel enzymatic extraction method for cAMP from Ziziphus jujuba fruit (jujube) paste. The paste was hydrolyzed at 50°C with 2.5 g/L pectinases (pectin lyase, cellulose, and hemicellulose, optimal ratio 4:2:1) for 3 h. The yield efficiency of jujube juice achieved 70%, and most importantly, the extracted cAMP content increased from 108 to 157 mg/kg. After resin adsorption, elution with formic acid, and filtration through a 150 Da nanofiltration membrane, the crude cAMP content was increased from 30% to 36% with the growing rate of 20% compared with the single pectinase treatment. The purification percentage of cAMP using silica gel column chromatography was as much as 97%.


Jujube, plant extract, cyclic adenosine monophosphate, silica gel column chromatography, nanofiltration, purification

Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Food and Health
Pages: 469-474

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :