Journal of Food, Agriculture and Environment

Vol 11, Issue 3&4,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255

A simple and convenient method for isolation of Lactobacillus


Sheng-ping Wang1,2#, Zhao-Hui Guo1#, Hui-Zhi Liu1*, De-Yuan Zhang1*, Yue-Lin He1, Yin-Hua Zhou1, Yan Zeng1, Xin-Xu Hu1, Shu-Feng Gao1, Xiao-Lin Zhou1, Yan Shu1, Li-Li Li2, Rui-Lin Huang2, Gang Liu2, Xia Xiong2

Recieved Date: 2013-07-11, Accepted Date: 2013-10-20


The aim of this study was to develop a convenient method for isolating Lactobacillus without anaerobic equipments. Glass bottles were used to contain the commercial MRS media supplemented with agar powder. After the media was dropped to the bottle, carbon dioxide (CO2) gas was aerated into the bottle, and then the bottle was autoclaved. The mouth of the bottle was sealed with sealing film and the bottle was placed obliquely until the media was solid. A bottle with solid slant medium was made for isolation of Lactobacillus. Using this new method, four different species of Lactobacillus were isolated from pig ilea digesta, which indicated that it is a simple and feasible method for Lactobacillus isolation.


Lactobacillus, isolation, simplified method

Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Food and Health
Pages: 564-565

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