Journal of Food, Agriculture and Environment

Point mutations in the viral genome-linked protein (VPg) of Potato virus Y probably correspond with ability to overcome resistance of tobacco

Author(s):

Marcin Przybys, Teresa Doroszewska, Apoloniusz Berbec

Recieved Date: 2013-07-20, Accepted Date: 2013-10-18

Abstract:

Potato virus Y (PVY) is a serious tobacco and potato pathogen that affects commercial crop production. In this paper, collection of 15 PVY isolates from tobacco plants, originating from Poland, Germany and Croatia, were characterized by using biological, serological and molecular methods. All these isolates were tested serologically using set of monoclonal antibodies. Ten isolates were classified to PVY^O-serotype, and five isolates to PVY^N-serotype. Some isolates of PVY were able to break resistance to PVY of the main tobacco resistance sources such as cultivars VAM and Wislica. The results of biological characterization of PVY isolates using susceptible and resistant tobacco varieties revealed that eight of PVY isolates broke the resistance of these cultivars. At the molecular level we investigated, whether the mutations within amino acid sequence of viral genome-linked protein (VPg) can influence the ability to break resistance in these varieties. Amino acids sequence analysis of VPg of fifteen PVY isolates revealed that amino acid residing at positions 101 and 105 was decisive in determining the ability to break the resistance of tobacco cultivars. The mutation in position 101, for the first time described in Polish isolates, was responsible for overcoming resistance to PVY in the varieties VAM and Wislica. This mutation involved substitution of serine in non-breaking isolates by glycine in resistance-breaking isolates. Moreover, the studies showed the breaking of resistance as a result of either of two mutations in position 105 of VPg protein, which led to replacing lysine or arginine by threonine or by glutamic acid.

Keywords:

Point mutation, VPg, Potyvirus, potato Virus Y, virulence, genomic sequence, serology, resistance, tobacco, amino acid sequence

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Agriculture
Pages: 986-989

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