Journal of Food, Agriculture and Environment
Effects of feeding on tobacco plants preinfested by Bemisia tabaci Biotype B on activity of detoxification enzymes in Trialeurodes vaporariorum
Author(s):
Haiyan Hu 1,
Ming Xue 1*, Mingjuan Bi 2, Xiao Zhang 1, Cheng Qu 1Recieved Date: 2013-07-10, Accepted Date: 2013-10-22
Abstract:Bemisia tabaci (Gennadius) biotype B is a serious invasive pest and widely distributed in China. B. tabaci biotype B and Trialeurodes vaporariorum (West wood) often coexist on greenhouse-grown vegetable crops and tobacco plant. Recent laboratory studies demonstrated that B. tabaci biotype B had the capacity to displace T. vaporariorum and B. tabaci biotype B now overlaps with T. vaporariorum where common hosts occur in most invaded field areas. The objective of this study was to define B. tabaci B-biotype-induced defense response of tobacco plants to T. vaporariorum and to explore its role in interspecific competition mechanism between B. tabaci and T. vaporariorum. We investigated the activity changes of detoxification enzymes in T. vaporariorum feeding on tobacco preinfested by Bemisia tabaci B-biotype with biochemical methods in laboratory. The results showed that T. vaporariorum adults and nymphs’ detoxification enzymes activities after feeding on systemic leaves preinfested by B. tabaci nymphs were significantly suppressed. The activity of carboxylesterase (CarE) (EC 3.1.1.1), glutathione S-transferase (GST) (EC 2.5.1.18) and cytochrome p450 monooxygenase (CyP450) (EC 1.14.14.1) in adults of T. vaporariorum was inhibited by 15.8%-41.8%, 25.5%-44.1% and 30.9%-47.3% after feeding on systemic leaves preinfested by B. tabaci nymphs from 6 h to 24 h compared with those in the controls, respectively. The activity of CarE, GST and CyP450 in 1st instar nymph was inhibited 33.9%, 36.4% and 21.3%, respectively, after feeding on systemic leaves preinfested by B. tabaci nymphs. The activities of CarE, GST and CyP450 in adults and nymphs of T. vaporariorum were slightly inhibited after feeding on local damaged leaves preinfested by B. tabaci nymphs, but only the activities of CarE in adults for 48 h and in 1st nymphs were significantly inhibited by 25.1% and 25.7% compared with those in the controls, respectively. Our results indicated that the detoxifying enzyme activities in adults and nymphs of T.vaporariorum were significantly suppressed after feeding on tobacco plant preinfested by B. tabaci nymphs concluding that it might play animportant role in B. tabaci displacing T. vaporariorum.
Keywords:Bemisia tabaci Biotype B,
Tobacco, Trialeurodes vaporariorum, preinfested, detoxifying enzymesJournal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Agriculture
Pages: 1029-1034
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