Journal of Food, Agriculture and Environment
Vol 11,
Issue 3&4,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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Identification of Phytophthora infestans from infected potato and tomato plants using molecular techniques
Author(s):
Wesam G. Shahrour 1*,
Mohamad A. Shatnawi 1, Samih Abubaker 2, Zakaria Al-Ajlouni 3, Ahmad N. Shadaideh 2, Hanee M. Al-Dmoor 4, Khalaf Alhussaen 5Recieved Date: 2013-06-14, Accepted Date: 2013-10-22
Abstract:Late blight caused by the oomycetes pathogen Phytophthora infestan is a devastating disease of potato and tomato worldwide. A total of 65 isolates of P. infestans were obtained from infected potato and tomato plants grown in different regions in Jordan within 2010-2012. DNA was extracted from pure culture of mycelium of the pathogen, prior it was amplified by PCR using specific primers of the β-tubulin gene, and subsequently they sequenced. The current study shows that PCR is very accurate method for specific detection of P. infestans in infected tomato and potato plants, this method will provide a useful tool for rapid detection from these plants. Sequences of the Jordanian isolates of this pathogen revealed a genetic variability between each other, and they show also a genetic variability with other published isolates in the world. They have 79-98% nucleotide identity between each other and 79-85% nucleotide identity with other isolates in the world like T30-4, haplotype H12, P10053, Can4, Dr98004, and P10124.
Keywords:Diversity,
DNA sequencing, late blight, Phytophthora infestans, potato, tomato, Tubulin geneJournal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Agriculture
Pages: 1216-1221
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