Journal of Food, Agriculture and Environment

Evaluation and selection of barley genotypes under optimum salt stress condition using tissue culture techniques and SDS-PAGE gel electrophoresis

Author(s):

Ehab Mohamed Rabei Metwali ^{1, 2*}, Michael Paul Fuller ³, Salah Mohamed Hassan Gowayed ^{1, 2}, Omar Abdel-Hakeim Almaghrabi¹, Yahia Yosef  Mosleh ^{1, 4}

Recieved Date: 2013-07-22, Accepted Date: 2013-10-28

Abstract:

Genetic analysis was studied in a 5 × 5 diallel cross following Hayman’s diallel analysis approach to obtain additive and dominance components of genetic variance using a mature embryo culture protocol. Different concentrations of NaCl at 5 and 10 g L^-1  were added to each medium. Callus fresh weight (g), shoot length (cm), root length (cm), proline (µg g^-1), sodium (mg g^-1) and potassium (mg g^-1) content were determined. Also, screening for non-water-soluble protein polymorphism was carried out using leaves derived via callus culture and  analysis using SDS-PAGE. Results on the genetic behaviour for previously traits, indicating the differences among the genotypes were significant (p ≤ 0.05) and highly significant (p ≤ 0.01). Wr/Vr graph in diallel analysis and average degree of dominance together with narrow-sense heritability values revealed additive gene effect for shoot length under control and saline treatments and callus fresh weight under control, while dominance gene action was detected for proline content under all treatments, K content under control and 5 g L^-1, callus fresh weight under 5 g L^-1 and root length under 10 g L^-1 NaCl. The order of array along the regression line showed that G.123 followed by G.124 are more stable than other parents and could be recommended for use in the production of a hybrid breeding programme for salt tolerance. Based on SDS-PAGE, approximately 36 bands which arranged between 124 kDa to 12 kDa were exhibited in most of parents and their hybrids under different treatments. Out of these 36 bands, 8 bands were polymorphic and one of these bands at 34 kDa was absent only in sensitive parent (Beecher) under salt treatments, while another band at 42 kDa appeared in most of the parents and their hybrids under salt treatments.

Keywords:

Barley (Hordeum vulgare L.), embryo culture, salinity, diallel analysis, hierarchical cluster analysis

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Agriculture
Pages: 1386-1394

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