Journal of Food, Agriculture and Environment

Differential gene expression in Primula under heat stress conditions

Author(s):

Weijuan Hu ^$, Lu Zhang ^$, Huitang Pan, Yike Gao, Qixiang Zhang *

Recieved Date: 2013-07-10, Accepted Date: 2013-10-14

Abstract:

Primula is a well-known alpine flower that is enjoyed as a garden plant because of its brilliant colours and distinctive flowers. High temperature is one of the key factors that limit the application of Primula because most plants in this genus are found at high altitudes and favour a cold environment. To gain insight into the molecular mechanisms of Primula in response to heat stress, a suppression subtractive hybridisation (SSH) cDNA library was constructed for a heat-tolerant species, P. forrestii, with mRNAs extracted from the leaves following treatment at 42°C for 2 hours. A total of 150 ESTs were generated, and the putative functions of these fragments were predicted by BLAST. These ESTs were found to belong to functional categories that include metabolism, chloroplast associated and photosynthesis, stress and defence, transcription factors, transporters and energy. Nearly 70% of the gene transcripts with known function had high homology with the heat shock protein (HSP) genes of other species. Among the total of 29 HSP genes, 73% of them were low-molecular-weight HSP genes according to their putative functions predicted by BLAST analysis. The expression of seven selected transcripts was examined by real-time quantitative PCR (RT-qPCR) using heat-treated leaves of P. forrestii. The results of RT-qPCR confirmed the differential expressions of these transcripts, and the expression of these genes was attributable to heat stress.

Keywords:

Primula, heat tolerance, differential gene expression, SSH

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Environment
Pages: 1642-1647

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