Journal of Food, Agriculture and Environment
Vol 11,
Issue 3&4,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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Ultrasound-assisted extraction of polysaccharides from the fruiting bodies of Tricholoma matsutake using response surface methodology
Author(s):
Qinghong You 1, 2, 3, 4,
Xiulian Yin 1, 3, 4*, Yuping Zhao 1Recieved Date: 2013-06-10, Accepted Date: 2013-09-30
Abstract:Ultrasonic technology was applied for polysaccharides extraction from the fruiting body of Tricholoma matsutake and response surface methodology (RSM) was used to optimize the effects of processing parameters on polysaccharides yields. On the basis of the results of a single-factors test, three independent and main variables, including extraction time (X1: 130-170 s), ultrasonic power (X2: 340-380 W) and ratio of water to raw material (X3: 45-55 ml/g) were studied by Box-Behnken design. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and also examined using the appropriate statistical methods. The best extraction conditions are as follows: extraction time 160 s, ultrasonic power 365 W and ratio of water to raw material 53.5 ml/g. Under the optimization conditions, the experimental yield of polysaccharides was 7.97 ± 0.31%, which was agreed closely with the predicted value (7.95%).
Keywords:Ultrasonic technology,
Tricholoma matsutake, response surface methodology, single-factors test, polysaccharidesJournal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Environment
Pages: 1969-1974
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