Journal of Food, Agriculture and Environment

Application of the response surface methodology for optimisation of biological nutrient removal from slaughterhouse effluents

Author(s):

Juliana Bortoli Rodrigues Mees ¹*, Simone Damasceno Gomes ², Sílvio César Sampaio ², Salah Din Mahmud Hasan ³, Gisele Maria Brod Caldereiro ¹, Cláudia Saramago de Carvalho Marques dos Santos Cordovil ⁴

Recieved Date: 2014-01-29, Accepted Date: 2014-03-30

Abstract:

The removal of nitrogen from wastewater is usually accomplished using biological conventional processes by nitrification plus denitrification, involving aerobic and anaerobic phases that are generally carried out in separate reactors or by different aeration intervals. The sequencing batch reactor (SBR) uses multiple steps in the same reactor, which include filling, reaction, settling, withdrawal and idling. The aim of the present work was to use experimental planning and response surface to assess the influence of the variables cycle time (CT) (8, 12, 13, 16, 19 and 20 h) and carbon:nitrogen ratio (C/N) (3, 4, 6, 8 and 9) in the biological removal of nitrogen from the effluents of a bird slaughterhouse and processing industrial facility using a sequencing batch reactor (SBR), as well as to optimise the process using central composite rotational design (CCRD) with four assays in levels -1 and +1, four assays on the axial points levels (-1.414 and +1.414) and five replicates at the central point (0) and validate the optimised condition. The nitrification process performance was evaluated by the efficiency of ammonia nitrogen removal (%), the denitrification process by the efficiencies of nitrite and nitrate removal (%) and the whole process by total inorganic nitrogen removal (%). The greatest values of removal of NH₄⁺-N (%) in the nitrification stage were obtained for CT of 16 h (C/N=9, CT of 19 h (C/N=8) and CT of 16 h (C/N=6) with removal values of 97.97%, 97.37% and 94.76%, respectively. In the denitrification stage, the greatest values of removal of NO₂^--N, 95.56% and 93.19% were obtained for CT of 16 h (C/N=3) and CT of 16 h (C/N=6), respectively, and the removal of NO₃^--N, 95.78% and 94.87% were obtained for CT of 16 h (C/N=6), CT of 19 h (C/N=4), respectively. In the whole process, the highest percentages of nitrogen removal were achieved with the cycle time of 16 h and C/N ratio of 6, validated experimentally, achieving an overall removal of inorganic nitrogen of  85.83±0.87%.

Keywords:

Sequencing batch reactor, central composite rotational design, nitrogen, nitrification, denitrification, poultry wastewater, wastewater treatment

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Journal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 2
Category: Environment
Pages: 786-792

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