Journal of Food, Agriculture and Environment

Assessment of clonal fidelity of micro-propagated Lagunaria patersonii plants by TRAP and SSR markers

Author(s):

Naglaa Mohamed Esmaiel ¹, Adel Ahmed Elshafei ^{2, 3}, Adel Moustafa Zakri ², Abdullah Abdlulaziz Al-Doss ², Mohamed Najeb Barakat ^{2, 4}*

Recieved Date: 2014-01-16, Accepted Date: 2014-03-29

Abstract:

A protocol is established for shoot regeneration of the threatened plants, Lagunaria patersonii, using lateral bud and shoot tip explants. Lateral bud and shoot tip explants were regenerated directly into shoots on Murashige and Skoog medium free growth regulators. The micro-propagated plantlets as well as mother plant were subjected to the target region amplification polymorphism (TRAP) and simple sequence repeat (SSR) analyses to assess the genetic stability of micro-propagated plantlets of Lagunaria. Twenty micro-propagated plantlets were chosen from a clonal collection of shoots that originated from a single mother plant. Out of 20 TRAP and 30 SSR primers screened, 20 TRAP and 28 SSR primers were found to produce clear, reproducible bands resulting in a total of 66 and 65 distinct bands, respectively, 93.2% and 73.4% were not polymorphic for TRAP and SSR analyses, respectively, among the Lagunaria patersonii and 20 in vitro generated clones. Based on the TRAP band data, similarity indicators between the progenies and the mother ranged from 0.95 to 1.0 and the polymorphic bands comprised 2.4% of the total scored genetic loci with TRAP analysis which indicates that this micro-propagated line of Lagunaria patersonii is genetically stable. A total of 1309 scorable bands were obtained from the full combination of SSR primers and plantlets and only 98 (7.49%) were polymorphic across the plantlets which indicated that the micro-propagated Lagunaria patersonii is genetically stable.

Keywords:

Genetic diversity, Lagunaria patersonii, in vitro culture, TRAP, SSR

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Journal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 2
Category: Environment
Pages: 916-921

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