Journal of Food, Agriculture and Environment
Vol 12,
Issue 2,2014
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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Inactivation effect of low pressure UV collimated beam irradiation on marine microalgae
Author(s):
Zi-Xi Wang 1*,
Xiang Xing 3, Wen-jun Sun 2*, Li-Qing Ma 1, Jing-Hui Zhao 4Recieved Date: 2014-01-18, Accepted Date: 2014-03-30
Abstract:Irradiation studies on 4 species of marine microalgae, namely Platymonas (Tetraselmis) helgolandica var. tsingtaoensis, Platymonas (Tetraselmis) subcordiformis, Dunaliella salina Teodoresce and Isochrysis galbana were performed using 0-500 mJ/cm2 UV quasi-parallel light. The activities of microalgae were evaluated by FDA-PI fluorescent double staining method, the cell number of microalgae were counted using fluorescence microscopy and flow cytometry, respectively, and the inactivation curves were formed. The results showed that Dunaliella salina Teodoresce and Isochrysis galbana had the poorest UV tolerance, which could be 100% inactivated with the UV doses of 250 and 350 mJ/cm2, respectively, Platymonas (Tetraselmis) subcordiformis had the strongest UV resistance ability, whose inactivation rate was lower than 37% with the highest dose of 500 mJ/ cm2, followed by Platymonas (Tetraselmis) helgolandica var. tsingtaoensis, that its inactivation rate was still below 81% with the same dose.
Keywords:UV parallel light,
microalgae, dose, fluorescent staining, flow cytometryJournal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 2
Category: Environment
Pages: 992-997
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