Journal of Food, Agriculture and Environment
Vol 12,
Issue 3&4,2014
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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Validation of ELISA-based detection of L. monocytogenes and E. coli O157:H7 in fresh cut vegetables
Author(s):
Marina Cavaiuolo 1,
Antonio Ferrante 1*, Spiros Paramithiotis 2, Agni Hadjilouka 2, Periklis Tzamalis 2, Eleftherios H. Drosinos 2Recieved Date: 2014-06-12, Accepted Date: 2014-09-20
Abstract:Innovative diagnostic methods were developed for the detection and quantification of Listeria monocytogenes and Escherichia coli O157:H7 in minimally processed fresh cut fruits and vegetables. The aim of the present study was to validate the technical efficiency of these methods and evaluate their efficacy and viability for routine analysis. To this purpose, ready-to-eat fresh fruits and vegetables were collected throughout the production chain. A multidisciplinary approach, including a newly developed ELISA method compared to ISO procedures, was applied to detect the pathogenic bacteria after harvesting, processing and shelf-life. Results obtained exhibited the technical efficiency of the developed methods showing similar sensitivity, specificity, negative predictive values and negative likelihood ratios.
Keywords:Leafy vegetables,
melon, vegetables, ELISA, ISOJournal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 3&4
Category: Food and Health
Pages: 98-99
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