Journal of Food, Agriculture and Environment

Vol 12, Issue 3&4,2014
Online ISSN: 1459-0263
Print ISSN: 1459-0255

In vitro regeneration of Acacia crassicarpa A. Cunn Ex Benth through organogenesis from juvenile sources


Griffin Akeng 1, Sures Kumar Muniandi 2, Nor Aini Ab Shukor 2, 3*

Recieved Date: 2014-06-12, Accepted Date: 2014-09-26


Micropropagation through tissue culture technique offers an alternative to traditional vegetative propagation to mass propagate selected trees for large-scale forest plantation. Therefore, this study aimed to develop a protocol for the micropropagation of A. crassicarpa. Nodal stem segment and leaf obtained from 2 month-old aseptically germinated seedlings were used as explant in this study. Nodal stem segment was found to be the most appropriate explant for shoot formation when cultured on a MS medium supplemented with 6-benzylaminopurine (BAP). The highest mean number of shoots (5) and the longest mean shoot elongation (8 mm) occurred on a medium supplemented with 0.5 mg l-1 BAP. The longest mean shoot length (8 mm) and the highest mean number of explants per culture (7) were obtained on medium without any plant growth regulator. When cultured on a medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D), nodal stem segment explant developed roots and callus (after 14 days). The highest mean number of roots (8.3 = 8) and the longest mean root length (12.0 = 12 mm) were obtained from the medium supplemented with 10.0 and 2.0 mg l-1 2,4-D, respectively. The highest mean number of roots (20.6 = 21) and the longest mean root length (10.4 = 10 mm) were obtained from the medium supplemented with 10.0 and 2.0 mgl-1 2,4-D, respectively, while the highest intensity of callus was produced on a medium supplemented with 8.0 and 10.0 mg l-1 2,4-D, and which was only able to produce root without any shoot formation. The calli produced were compact, watery and white in colour. Survival rate of plantlets was higher (100%) when transferred into the autoclaved mixture of soil, sand and peat (3:3:1) than those transplanted in an unautoclaved soil mixture (6.6%). Survival percentages of the plantlets in the culture room and greenhouse condition were 85 and 100%, respectively.


A. crassicarpa, micropropagation, organogenesis, plant growth regulator, cytokinin, auxin, leaf segment, nodal segment, Murashige and Skoog’s medium (MS), in vitro rooting, in vitro shooting, acclimatisation

Journal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 3&4
Category: Environment
Pages: 375-382

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :