Journal of Food, Agriculture and Environment

Isolation, screening and characterization of new PGPR isolates from rhizosphere of alfalfa plants


Abdullah S. Alsohim 1,2, Ramadan Almohamad 1,  M. I. Motawei 1,2*

Recieved Date: 2018-01-10, Accepted Date: 2018-03-26


Plant growth-promoting rhizobacteria (PGPR) can be successfully used as biofertilizers for many crops.  PGPR, as a group of PGPB colonizing root surface, are widely studied. This study aimed to identify the PGPR isolates by 16S rDNA sequencing and to evaluate the bacteria for PGPR traits. Further, selected isolates were evaluated for their ability to enhance alfalfa plant growth.  Twenty five soil samples were collected from the rhizosphere around alfalfa plants in different areas in Al-Qassim Region. Based on 16S rDNA gene sequencing, these bacterial strains were identified under several genera: Sinorhizobium, Acinetobacter, Pantoea, Stenotrophomonas, Klebsiella, Serratia, Kluyvera, and Enterobacter. In a total of 6 Sinorhizobium isolates, just 2 isolates (SN2 and SN4) showed the highest phosphate solubilizition efficiency. Only one isolate, Sinorhizobium sp. isolate SN2, was a very good siderophore producer according to this qualitative assay. The isolates SN1, SN2, and SN3 gave low IAA production. Moreover, the results revealed that Sinorhizobium meliloti strain SN4 significantly increased shoot and root weight of alfalfa plants. These results suggest that some Sinorhizobium bacterial strains may be useful as biofertilizers in alfalfa.


PGPR,  Sinorhizobium sp., alfalfa

Journal: Journal of Food, Agriculture and Environment
Year: 2018
Volume: 16
Issue: 2
Category: Agriculture
Pages: 81-85

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