Journal of Food, Agriculture and Environment

A standardized protocol for the genomic DNA isolation from the leaf of Camellia sinensis (Linn.) O. Kuntze


Ananya Phukan 1, Jyotsna Gayan 2, Aswathy Ravindran 1,  Bandana Nabis 1*

Recieved Date: 2018-08-08, Accepted Date: 2018-09-22


Relatively high amount of DNA could be extracted from the leaf sample of Camellia sinensis (Linn.) O. Kuntze by following the standardized and modified protocol. Isolated DNA was quantified using a spectrophotometer at the absorbance of λ260  and λ280 nm. Tea being an important commercial crop in India, many research works has been done to improve tea quality and yield. Clonal varieties have been developed which have considerable genetic variability. Eight clonal varieties of Camellia sinensis (Linn.) O. Kuntze were selected from various tea gardens of Golaghat district, one of the major tea producing districts of Assam, India.  The collected clonal varieties were all produced in the Tocklai Tea Research Institute, Assam. All the clonal varieties yielded a good quality DNA in a range of 1.81-2.77 mg/ml  and produced clear bands on the agarose gel. The estimation of purity of the obtained DNA was concluded by analyzing the ratio of λ260 nm/λ280 nm of the genomic DNA, which ranged 1.93-2.73. The obtained DNA was then statistically analyzed for concentration and purity, which showed considerable differences.


Genomic DNA isolation, standardized protocol,  Camellia sinensis, clonal varieties

Journal: Journal of Food, Agriculture and Environment
Year: 2018
Volume: 16
Issue: 3&4
Category: Agriculture
Pages: 33-37

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers / registered users with an online subscription to this Journal. If you have set up a personal subscription to this title please enter your user name and password ( All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, visit the site Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :