Journal of Food, Agriculture and Environment

Isolation and screening of microorganisms from traditional and modified agro-organic formulations

Author(s):

Smita Jadhav, Sunita Singh, Arpita Gupte

Recieved Date: 2021-10-10, Accepted Date: 2021-12-08

Abstract:

Agriculture is an important sector contributing to the Indian economy. Adopting eco-friendly approaches should be preferred over chemical fertilizers due to their cost-effectiveness, better crop productivity, and sustainability. The use of native organic preparations like Panchagavya and Jeevamrit which are rich in macronutrients, micronutrients, plant growth-promoting substances, and beneficial microorganisms are proved to be promising in achieving sustainable agriculture. The objective of this study is to isolate and characterize the plant growth-promoting abilities of the microorganisms from the traditional and modified formulations prepared using agro-organic waste. Four different formulations viz., traditional and modified Panchagavya and Jeevamrit were respectively prepared. The microbial population present in these formulations was evaluated for microbial load by enumerating the number of bacteria, fungi, and actinomycetes at different time intervals of preparation. Among the traditional formulations, the highest microbial population was observed in Jeevamrit (19.1×108 CFU/ml) followed by Panchagavya (3.04×108 CFU/ml). While for modified formulations, the highest microbial population was observed in modified Jeevamrit (11.41×108 CFU/ml) followed by modified Panchagavya (1.72×108 CFU/ml). The study also attempted to estimate, the percentage of nitrogen fixers and phosphate solubilizers in both traditional and modified formulations with respect to time. A common trend of increasing percentage of nitrogen fixers and phosphate solubilizers was observed in all the prepared formulations. A total of 105 different microbial isolates were isolated. For isolating bacteria from this agro-organic preparation, nystatin (50 mg/l) was used to inhibit the growth of fungus. Among the 105 isolates, the Gram character of the 68 bacterial isolates was confirmed by staining and non-staining (KOH) methods. The isolates were further screened qualitatively for different metabolic characteristics like nitrogen fixation, phosphate solubilization, starch hydrolysis, cellulose, and pectin degradation. Of these, 11 bacterial isolates showing plant growth-promoting activities like atmospheric nitrogen fixation and phosphate solubilization were selected for further studies.

Keywords:

Organic formulations, Panchagavya, Jeevamrit, nitrogen fixers, phosphate solubilizers, plant growth promotion

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Journal: Journal of Food, Agriculture and Environment
Year: 2022
Volume: 20
Issue: 1
Category: Agriculture
Pages: 87-91

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