Journal of Food, Agriculture and Environment




Monitoring mutagenic effectiveness and efficiency of gamma rays and sodium azide in breeding line of linseed: Implicating their roles in induction of chlorophyll mutations


Author(s):

Roshan Jahan, Ruhul Amin, Samiullah Khan

Recieved Date: 2020-10-02, Accepted Date: 2020-12-15

Abstract:

Since many decades, the plant breeders are enthusiastically engaged for utilizing the useful mutations for plant breeding and the systematic study of induced mutagenesis is vital in order to produce the genetic diversity in a variety of crops. Previously, several similar studies have been undertaken for many cereal crops. Gamma rays and sodium azide were used to induce the chlorophyll mutations in a variety of linseed, Padmini. The seeds were treated with four doses of gamma rays (50 Gy, 100 Gy, 150 Gy and 200 Gy) and four doses of sodium azide (0.1% SA, 0.2% SA, 0.3% SA and 0.4% SA). The combined treatments of gamma rays and sodium azide (50 Gy +0.1% SA, 100 Gy+0.2% SA, 150 Gy+0.3% SA and 200 Gy+0.4% SA) were also given to compare their effectiveness. Chlorophyll mutants were found in M2 generation when seedlings were 15-25 days old. In mutation breeding the basic parameters considered for the assessment of mutagenic potency of the mutagens are their effectiveness and efficiency. In view of their effectiveness and efficiency, frequency and spectrum of mutations are discussed. Therefore, we observed a broad spectrum of chlorophyll mutations in M2 generation. In the present study, maculata and xanthaviridis survived up to the maturity, however, albino, chlorina, tigrina and xantha were found dead at the seedling stage. The highest frequency of chlorophyll mutations was perceived in combined treatments of gamma rays and sodium azide in contrast to their individual treatments. The effectiveness and efficiency were estimated based on chlorophyll mutations frequency. The main objective of the induced mutagenesis is to increase the yield of the several crops. However, linseed has been identified as a health improving plant and used in many diseases. The importance of linseed is epitomized by its various uses in daily life as human food, animal feed and industrial applications.

Keywords:

Chlorophyll mutations, gamma rays, linseed, mutagenic effectiveness and efficiency, sodium azide


Journal: Journal of Food, Agriculture and Environment
Year: 2021
Volume: 19
Issue: 1
Category: Agriculture
Pages: 61-67


Full text for Subscribers
Information:

Note to users

The requested document is freely available only to subscribers / registered users with an online subscription to this Journal. If you have set up a personal subscription to this title please enter your user name and password (https://www.wflpublisher.com/Pages/subscription-procedure). All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, visit the site https://www.wflpublisher.com/Journal. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.


Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :