Journal of Food, Agriculture and Environment

Monitoring mutagenic effectiveness and efficiency of gamma rays and sodium azide in breeding line of linseed: Implicating their roles in induction of chlorophyll mutations

Author(s):

Roshan Jahan, Ruhul Amin, Samiullah Khan

Recieved Date: 2020-10-02, Accepted Date: 2020-12-15

Abstract:

Since many decades, the plant breeders are enthusiastically engaged for utilizing the useful mutations for plant breeding and the systematic study of induced mutagenesis is vital in order to produce the genetic diversity in a variety of crops. Previously, several similar studies have been undertaken for many cereal crops. Gamma rays and sodium azide were used to induce the chlorophyll mutations in a variety of linseed, Padmini. The seeds were treated with four doses of gamma rays (50 Gy, 100 Gy, 150 Gy and 200 Gy) and four doses of sodium azide (0.1% SA, 0.2% SA, 0.3% SA and 0.4% SA). The combined treatments of gamma rays and sodium azide (50 Gy +0.1% SA, 100 Gy+0.2% SA, 150 Gy+0.3% SA and 200 Gy+0.4% SA) were also given to compare their effectiveness. Chlorophyll mutants were found in M2 generation when seedlings were 15-25 days old. In mutation breeding the basic parameters considered for the assessment of mutagenic potency of the mutagens are their effectiveness and efficiency. In view of their effectiveness and efficiency, frequency and spectrum of mutations are discussed. Therefore, we observed a broad spectrum of chlorophyll mutations in M2 generation. In the present study, maculata and xanthaviridis survived up to the maturity, however, albino, chlorina, tigrina and xantha were found dead at the seedling stage. The highest frequency of chlorophyll mutations was perceived in combined treatments of gamma rays and sodium azide in contrast to their individual treatments. The effectiveness and efficiency were estimated based on chlorophyll mutations frequency. The main objective of the induced mutagenesis is to increase the yield of the several crops. However, linseed has been identified as a health improving plant and used in many diseases. The importance of linseed is epitomized by its various uses in daily life as human food, animal feed and industrial applications.

Keywords:

Chlorophyll mutations, gamma rays, linseed, mutagenic effectiveness and efficiency, sodium azide

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Journal: Journal of Food, Agriculture and Environment
Year: 2021
Volume: 19
Issue: 1
Category: Agriculture
Pages: 61-67

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