Journal of Food, Agriculture and Environment

Vol 3, Issue 2,2005
Online ISSN: 1459-0263
Print ISSN: 1459-0255

A simple protein extraction method for proteomic studies on olive leaves


Nasir S. A. Malik *, Joe M. Bradford

Recieved Date: 2005-01-12, Accepted Date: 2005-03-10


Researchers have encountered difficulties in extracting proteins from olive leaves because of the high content of interfering compounds in this tissue. Recently, after testing several methods, a phenol-SDS method was devised to extract proteins from olive leaves for proteomic studies. This method is tricky and the use of phenol, a very corrosive agent, requires careful handling. We therefore tested the phenol-SDS method along with a slightly modified standard method that gave equal, or better, results on two dimensional electrophoresis gels. This simpler and more convenient method of protein extraction from olive leaves is described here.


Protein extraction, olive leaves, proteomics, 2-D electrophoresis

Journal: Journal of Food, Agriculture and Environment
Year: 2005
Volume: 3
Issue: 2
Category: Agriculture
Pages: 246-248

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :