Journal of Food, Agriculture and Environment

Vol 4, Issue 2,2006
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Chemical composition, antibacterial and antimutagenic activities of four populations of Rosmarinus officinalis L. oils from Tunisia


Zohra Marzouk 1*, Hédi Ben Mansour 2, 7, Imed Chraief 3, Ridha Mosrati 7, Jihene Cheriaa 4, Aicha Neffati 2, Belsem Marzouk 4, Mounira Sfari 4, Kamel Boukef 5, Daniel Barillies 7, Leila Chekir Ghedira 2, 6

Recieved Date: 2005-12-18, Accepted Date: 2006-03-25


The first aim of this study was to describe in detail the chemical composition of four Tunisian samples of Rosmarinus officinalis L. essential oils (EOs) from Matmata (S1), Mahdia (S2), Monastir (S3) and Elkram (S4). The second aim was to test their antibacterial and their antimutagenic activities. The isolation of leaves EOs was done by hydrodistillation using a Clevenger-type apparatus. The chemical analysis was carried by using gas chromatography and mass spectrometry (GC-MS). The antimicrobial activity was evaluated against five pathogenic bacteria: Gram-negative strains Escherichia coli and Pseudomonas aeruginosa and Gram-positive strains Staphylococcus aureusStaphylococcus epidermidis and Micrococcus luteus. All the EOs were dissolved in Tween 80. The minimum inhibitory concentrations (MIC) and the minimum bactericidal concentrations (MBC) were determinated by sub-culture at TSA agar plates which were incubated at 37°C during 18-24 h. Forty compounds were identified. The S1 oils were characterised by a higher content of α-pinene and camphene (11.3 and 10.7% respectively). The compound 1,8-cineole was most represented in S2 (46.1%) and in S3 (46.6%) while rosemary oils of S1 and S4 had approximately the same content (25.1% and 24.1% respectively). S4 sample had the highest level of camphor (36.9%). S4 and S3 samples had the highest antimicrobial activity against all bacteria tested; E. coli was the most sensitive to S3 EOs. They can be suggested as the best antimutagenic agents. The quantitative composition of EOs differs between geographical sources. The antibacterial and antimutagenic activities are not related only to the major compounds but also to the minor components of rosemary EOs. These oils can be used as an antimicrobial agent in hospitals by pulverisation or in soaps and in foods such as fish, meat, milk and its derives. Why not use regularly honey of bees which visit always rosemary, to treat chronic bacterial infections or cancer?


Rosmarinus officinalis L., chemotype, essential oil composition, antibacterial activity, mutagenicity, antimutagenicity, Ames test

Journal: Journal of Food, Agriculture and Environment
Year: 2006
Volume: 4
Issue: 2
Category: Food and Health
Pages: 89-94

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :