Journal of Food, Agriculture and Environment

Vol 4, Issue 2,2006
Online ISSN: 1459-0263
Print ISSN: 1459-0255

In vitro zygotic embryo germination of Calamus latifolius Roxb. and Calamus tenuis Roxb.


Bharati Meitram, G. J. Sharma *

Recieved Date: 2005-11-27, Accepted Date: 2006-03-18


A highly economical medium for in vitro zygotic embryo germination of two priority species of rattan (Calamus latifolius Roxb. and Calamus tenuis Roxb.) has been used for the first time. Seedling growth of embryo explants in three different media, namely, Murashige and Skoog medium (MS), woody plant medium (WPM) and Y3 medium supplemented with plant growth regulators (PGRs) was investigated. Embryo explants inoculated onto WPM supplemented with various concentrations of PGRs produced increased seedling growth compared to the other two media used. Benzylaminopurine (BAP), 4.54-8.88 µM, produced higher shoot length when treated singly than in combination with 0.54-5.57 µM α-naphthalene acid (NAA). Root length was found to be highest in 0.54 µM NAA supplemented WPM in both the species. Combination with BAP produced no significant increase in root length. Multiple shoot induction occurred best on WPM supplemented with a combination of 23.25 µM kinetin (Kn) and 2.26 µM 2,4-dichlorophenoxyacetic acid (2,4-D). Complete plantlet development occurred faster in WPM in both the species.


In vitro propagation, priority species, Calamus latifolius Roxb., Calamus tenuis Roxb.

Journal: Journal of Food, Agriculture and Environment
Year: 2006
Volume: 4
Issue: 2
Category: Environment
Pages: 306-309

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :