Journal of Food, Agriculture and Environment

Vol 8, Issue 1,2010
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Effect of nitrogen fertilization on ascorbic acid, glucoraphanin content and quinone reductase activity in broccoli floret and stem


Chao-Jiong Xu, Rong-Fang Guo, Hui-Zhuan Yan, Jing Yuan, Bo Sun, Gao-Feng Yuan, Qiao-Mei Wang *

Recieved Date: 2009-09-16, Accepted Date: 2009-12-30


The effect of five levels of nitrogen fertilization (N 0, 100, 200, 300 and 400 kg ha-1) on head size, shelf life, concentrations of ascorbic acid, glucoraphanin and quinone reductase (QR) activity of broccoli (Brassica oleracea var. italica) was investigated in two broccoli cultivars, Youxiu and Lvling. The results showed that both the average weight and diameter of main head were significantly increased with the increase of nitrogen fertilization in both cultivars. However, the ascorbic acid and glucoraphanin concentrations in broccoli floret and stem were decreased with the increase in nitrogen application, and significant lower levels of ascorbic acid were detected by nitrogen fertilization at range of N 300-400 kg ha-1. The nitrogen fertilization at N 400 kg ha -1 also significantly reduced the shelf life of broccoli as well as glucoraphanin concentration in broccoli floret and stem. The QR activity in broccoli floret and stem supplied with different nitrogen paralleled their glucoraphanin concentrations. In conclusion, moderate nitrogen fertilization rate (N 200-300 kg ha-1) could significantly increase the head size and maintain the storage life, glucoraphanin concentration and QR activity.


Nitrogen fertilization, glucoraphanin, quinone reductase activity, glucosinolate, head size, shelf life

Journal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 1
Category: Agriculture
Pages: 179-184

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :