Journal of Food, Agriculture and Environment

Vol 8, Issue 2,2010
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Five-minute purification of PCR products by new-freeze-squeeze method


Jin-Long Yang 1, 2, Rui Yang 1, An-Chun Cheng 2, 3*, Ren-Yong Jia 2, Ming-Shu Wang 2, Su-Hui Zhang 1

Recieved Date: 2010-01-18, Accepted Date: 2010-04-22


Here, we describe a new-freeze method (NFM) for rapid purification of PCR products. After PCR products were separated by horizontal common agarose electrophoresis, gel fragments containing the desired PCR products were directly subjected to NFM. The resulting gel was frozen in nitrogen canister for 60 s and thawed in 70°C for 2 min and then centrifuged. DNA in the supernatant was stored at -20°C until further use. PCR products in the size range of 100-2000 bp were purified within nearly 5 min. NFM could separate 2 DNA fragments that differed in length by 50 bp. As judged by the purification efficiency, the quality of PCR products separated by NFM was equal to that of the method of DNA purification by low-melting-agarose gel method (LMM) and freeze-squeeze method (FSM). NFM reduces the operational time and cost of recombinant DNA technology, in which purification of PCR products is a repetitive process. With no complicated equipment and technical training, NFM is very simple and easy to operate.


Agarose gel electrophoresis, new-freeze method, PCR, purification

Journal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 2
Category: Food and Health
Pages: 32-33

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