Journal of Food, Agriculture and Environment

Five-minute purification of PCR products by new-freeze-squeeze method

Author(s):

Jin-Long Yang ^{1, 2}, Rui Yang ¹, An-Chun Cheng ^{2, 3*}, Ren-Yong Jia ², Ming-Shu Wang ², Su-Hui Zhang ¹

Recieved Date: 2010-01-18, Accepted Date: 2010-04-22

Abstract:

Here, we describe a new-freeze method (NFM) for rapid purification of PCR products. After PCR products were separated by horizontal common agarose electrophoresis, gel fragments containing the desired PCR products were directly subjected to NFM. The resulting gel was frozen in nitrogen canister for 60 s and thawed in 70°C for 2 min and then centrifuged. DNA in the supernatant was stored at -20°C until further use. PCR products in the size range of 100-2000 bp were purified within nearly 5 min. NFM could separate 2 DNA fragments that differed in length by 50 bp. As judged by the purification efficiency, the quality of PCR products separated by NFM was equal to that of the method of DNA purification by low-melting-agarose gel method (LMM) and freeze-squeeze method (FSM). NFM reduces the operational time and cost of recombinant DNA technology, in which purification of PCR products is a repetitive process. With no complicated equipment and technical training, NFM is very simple and easy to operate.

Keywords:

Agarose gel electrophoresis, new-freeze method, PCR, purification

---

Journal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 2
Category: Food and Health
Pages: 32-33

---

Full text for Subscribers

---

Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

---

Purchase this Article:   20 Purchase PDF Order Reprints for 15

---

Share this article :