Journal of Food, Agriculture and Environment

Regulation of endogenous hormones on post-harvest senescence in transgenic broccoli carrying an antisense or a sense BO-ACO 2 gene

Author(s):

Feifei Qin ^{1, 2, 3}, Cheng-rong Wang ¹, Ran Wang ^1*, Gang Ma ¹, Hui-lian Xu ³

Recieved Date: 2008-11-15, Accepted Date: 2009-04-14

Abstract:

It has been confirmed by our previous study that the transgenic broccoli with a sense (S line) or an antisense (AS line) BO-ACO 2 gene shows delayed postharvest senescence. Since endogenous phytohormones play the main role in plant senescence, it is important to elucidate how phytohormones change and affect the postharvest senescence in transgenic broccoli. Therefore, in this study, the regulation effects of endogenous ethylene, abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellic acid (GA₃) and zeatin riboside (ZR) on immaturity senescence were analyzed in postharvest transgenic broccoli heads. The delayed onset of floret yellowing in transgenic broccoli was mainly attributed to reduced ethylene production. Compared with the earlier senescence in control, transgenic broccoli showed increasing concentration of ZR during the storage time and reduced levels of ABA and IAA in florets. GA₃ was one of the possible factors for almost 5-day storage life in AS line but seemed not crucial for floret senescence in the S line. In conclusion, BO-ACO 2 gene was directly involved in ethylene biosynthesis and at the same time regulated the metabolisms of plant hormones ABA, IAA, GA₃ and ZR. The rebalancing of mechanism of plant hormones in florets of transgenic broccoli due to the integrated BO-ACO 2 gene was closely associated with the retardation of postharvest senescence.

Keywords:

Abscisic acid, gibberellic acid, indole-3-acetic acid, senescence, transgenic broccoli, zeatin riboside

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Journal: Journal of Food, Agriculture and Environment
Year: 2009
Volume: 7
Issue: 2
Category: Agriculture
Pages: 594-598

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