Journal of Food, Agriculture and Environment

Evaluation of genetic divergence among traditional accessions of common bean by using RAPD molecular markers

Author(s):

Marco Antonio A. Barelli ¹, Juliana P. Poletine ^2*, Cláudia Thomazella ³, Pedro S. Vidigal Filho ², Elieser Souto ², Cinthya M. N. A. Pacheco ², Leonarda Grillo Neves ¹, Julio César Ferreira Elias ¹, Maria Celeste Gonçalves-Vidigal ²

Recieved Date: 2011-01-18, Accepted Date: 2011-03-29

Abstract:

The objective of this study was to evaluate genetic divergence among 30 traditional cultivars of common bean from Mato Grosso do Sul State (Brazil) by using RAPD molecular markers. Genetic diversity among accessions was evaluated by DNA electrophoretic banding patterns; presence or absence of bands (coded 1 or 0, respectively) provided data for constructing a similarity matrix based on Jaccard’s coefficient. This matrix was converted to an inverse dissimilarity matrix, allowing a cluster analysis (using the hierarchical nearest neighbor procedure) of traditional accessions. RAPD analyses produced 97 DNA bands from the 30 accessions, and cluster analysis demonstrated the presence of four distinct groups. The first group was subdivided into three classes, of which only one was of Andean origin. Mulatinho Vagem Roxa B and Jalo accessions were the most divergent (91.80%), while Rosinha B and Rosinha Opaco were the most similar (18.67%). The precise genetic divergence pattern that was uncovered allowed to distinguish Andean and Mesoamerican genotypes. Therefore, the best strategy to be adopted is the use of the combination Mulatinho Vagem Roxa B x Jalo in interpopulational breeding program in order to obtain superior segregants.

Keywords:

Traditional cultivars, Phaseolus vulgaris L., genetic diversity, molecular markers

---

Journal: Journal of Food, Agriculture and Environment
Year: 2011
Volume: 9
Issue: 2
Category: Agriculture
Pages: 195-199

---

Full text for Subscribers

---

Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

---

Purchase this Article:   20 Purchase PDF Order Reprints for 15

---

Share this article :