Journal of Food, Agriculture and Environment

In vitro digestion/centrifugal ultrafiltration to determine the oral bioavailability of lead in soils

Author(s):

Xiaochen Chen, Jia Niu and Yanshan Cui *

Recieved Date: 2011-11-18, Accepted Date: 2012-01-03

Abstract:

Lead (Pb) is a common environmental contaminant found in soils. The oral bioavailability of soil Pb for humans depends on the ability of the stomach and the small intestine to dissolve the soil Pb (bioaccessibility) and on the ability of the intestinal membranes to absorb the contaminant. In vivo and in vitro methods are often used to estimate of oral bioavailability of Pb in soil. As a cheap, simple and reproducible tool, in vitro methods based on human physiology have become prevalent. Many in vitro digestion models have been developed during the past few decades, but they are still not perfect. In this study, in vitro digestion and 10 kD centrifugal ultrafiltration device were used to estimate the oral bioavailability and bioaccessibility of Pb in 5 soil samples. The three soil samples were collected from Guangdong (a paddy field near mining area), Guangxi (a cropland) and Neimenggu (a mining area) of China, and other two soil samples, GSS-1 and GSS-5, were Chinese standard reference soils, which represented the typical soils in north and south China. The 5 soil samples cover a range of Pb concentrations from 98 to 5860 mg kg^-1. Results showed that not only the bioaccessibility of Pb, but also the ratio of Pb bioavailability to bioaccessibility varied with the soil samples. The bioaccessible and bioavailable Pb fraction range was 16.10-81.92% and 15.23-80.18% in the gastric phase and 1.69-32.72% and 0.93-20.62% in the small intestinal phase, respectively. The ratio of Pb bioavailability to bioaccessibility ranged from 0.76 to 0.99 and from 0.24 to 0.80 in the gastric and small intestinal phase, respectively. In vitro digestion/centrifugal ultrafiltration is feasible and easy to operate to assess the bioaccessibility and bioavailability of soil heavy metals.

Keywords:

Lead, soil, in vitro method, bioaccessibility, bioavailability, centrifugal ultrafiltration

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Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 1
Category: Environment
Pages: 681-684

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