Journal of Food, Agriculture and Environment

Vol 10, Issue 3&4,2012
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Transport of traditional Chinese pimple milk-derived angiotensin-converting enzyme (ACE) inhibitory peptides across a Caco-2 cell monolayer and their molecular recognition with ACE


Cuie Guang *,  Jiangang Shang, Bo Jiang

Recieved Date: 2012-08-02, Accepted Date: 2012-09-24


A Caco-2 cell monolayer was used to predict the transport of bioactive peptides in human intestine. Three di-peptides from the digest of traditional Chinese pimple milk that were transportable across the monolayer with high content and strong ACE inhibitory ability were determined to be Val- Pro, Lys-Pro and Tyr-Pro. The same order of magnitude for apparent permeability coefficients (1.8×10-6, 2.2×10-6  and 2.6×10-6 cm·s-1, respectively) revealed the similar transport capacity of the three peptides. For Km values, Lys-Pro (24.4 mM) and Tyr-Pro (17.5 mM) exhibited higher affinity toward the transport compared with Val-Pro (42.7 mM). Automated molecular docking showed a lower affinity energy for the best pose of Lys-Pro (-47.30 kJ/mol) at the ACE active site than for those of Val-Pro (-34.74 kJ/mol) and Tyr-Pro (-32.78 kJ/mol). The interactions, including H-bonds, hydrophobic, hydrophilic and electrostatic interactions, as well as the interaction with Zn2+, were responsible for the binding of inhibitory peptides to ACE. 


Caco-2 cell monolayer, transport kinetics, molecular docking, traditional Chinese pimple milk, ACE inhibitory peptides

Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Food and Health
Pages: 40-44

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