Journal of Food, Agriculture and Environment

Vol 10, Issue 3&4,2012
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Genetic variation in 27 accessions of Arachis genus using target region amplification polymorphism markers


Li-Xian Qiao, Lei Xu, He-Chun Wang, Jiong-Ming Sui, Jing-Shan Wang

Recieved Date: 2012-06-29, Accepted Date: 2012-09-30


Target region amplification polymorphism (TRAP) technique aims for the amplification of open reading frames (ORFs) by use of an arbitrary primer and a fixed primer. The TRAP analytic system was set up and successfully used in Arachis genus in this study. Seventeen cultivars of peanut and 10 wild relatives were screened by the TRAP analytic system with 50 primer combinations, 20 of which produced stable and reproducible amplification patterns in three repeated experiments. The 20 primer combinations produced 460 amplified fragments, 410 of which (89.13%) were polymorphic, with an average of 20.5 polymorphic fragments for each primer combination, ranging in size from 50 bp to 1500 bp. The 460 fragments were scored one by one and then used to develop a dendrogram of the 27 accessions of Arachis genus with unweighted pair-group method arithmetic average (UPGMA). The genetic distance among these accessions ranged from 0.03 to 0.46. These accessions were divided into three major groups at the 0.69 similarity level: all the 17 cultivated peanuts, the 9 wild relatives apart from No. 18 and the wild relative No. 18 itself. One of the specific markers, DGAT/GA3-18264 from A. villosa B., was successfully converted into sequence characterized amplification region (SCAR) marker. The TRAP technique was a simple and available molecular marker technique and might be used in the diversity detection of Arachis


Arachis, genetic variation, molecular marker, peanut, TRAP

Journal: Journal of Food, Agriculture and Environment
Year: 2012
Volume: 10
Issue: 3&4
Category: Agriculture
Pages: 639-644

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