Journal of Food, Agriculture and Environment

Detection of chronic and acute bee paralysis viruses from Jordanian honeybee apiaries by reverse transcriptase PCR

Author(s):

Amal A. Al-Abbadi ^1*, Dhia S. Hassawi ², Saeid Abu-Romman ², Saida A. Abu-Mallouh ²

Recieved Date: 2010-07-06, Accepted Date: 2010-10-27

Abstract:

The occurrence and distribution pattern of chronic and acute bee paralysis viruses (CBPV and ABPV, respectively) were investigated in 100 Jordanian honeybee colonies distributed throughout the area. Honeybee samples were apparently healthy and/or suffering from symptoms of depopulation, sudden collapse, paralysis or dark coloring. Reverse transcriptase PCR technique was used to detect the presence of these two viruses in Jordan. The most prevalent virus was CBPV, present in 12% of samples, while ABPV was present in 8% samples. The distribution pattern of both viruses varied considerably in the different investigated geographical regions of Jordan apiaries. Honeybee samples for ABPV virus obtained from apparently healthy colonies gave higher infestation percentage with 62.5% in comparison with the symptomatic samples with 37.5%. On the other hand, in case of CBPV, problematic samples gave higher infestation level with 58.3% in comparison with 41.7% in apparently healthy samples. In some samples, simultaneous infections of the two viruses were observed. The size of the RT-PCR product for ABPV was (721) nucleotides and found to be 97% identical to the Gene Bank, and the size of the RT-PCR product for CBPV was (455) nucleotides. This is the first record for the presence of ABPV in Jordan. This study indicated that RT-PCR technique facilitates the identification of honeybee viruses at molecular level.

Keywords:

Apis mellifera, honeybee viruses, chronic bee paralysis virus, acute bee paralysis virus, RT-PCR

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Journal: Journal of Food, Agriculture and Environment
Year: 2010
Volume: 8
Issue: 3&4
Category: Environment
Pages: 1016-1019

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