Journal of Food, Agriculture and Environment
Vol 11,
Issue 1,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255
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Quantitative determination of polyphenols in tobacco leaves by HPLC
Author(s):
Xiaoming Ji,
Yaowei Wei, Guoshun Liu *, Hongli ChenRecieved Date: 2012-09-20, Accepted Date: 2013-01-26
Abstract:A high performance liquid chromatographic (HPLC) method for the determination of 9 polyphenols in tobacco was studied. These polyphenols were extracted from tobacco sample by ultrasonic extraction method. Polyphenols were separated on a Waters Sunfire C18 (4.6 mm ×150 mm, 5 µm) chromatographic column. The mobile phase was methanol, water and 2% HAC solution on gradient elution at a flow rate of 1 ml/min. Each polyphenol was monitored by UV detector and 9 polyphenols in tobacco could be simultaneously determined by this method. The recovery of these polyphenols was 94~ 99%, and relative standard deviation from 0.44% to 4.26%. This method was applied to the determination of polyphenols in tobacco with good results.
Keywords:Tobacco,
ultrasonic extraction, HPLC, polyphenolsJournal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 1
Category: Environment
Pages: 868-870
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