Journal of Food, Agriculture and Environment

An assessment of in vitro culture and plant regeneration from leaf base explants in carnation (Dianthus caryophyllus L.) 

Author(s):

Naglaa M. Esmaiel ¹, Abdullah Abdlulaziz Al-Doss ², Mohamed Najeb Barakat ^{2, 3}*

Recieved Date: 2012-09-20, Accepted Date: 2013-01-18

Abstract:

Ten cultivars of carnation (Dianthus caryophyllus L.) were screened by using leaf base explants and four media, for their capability of callus induction, embryogenic callus and plant regeneration. The results indicated that all in vitro culture traits were significantly influenced by the differences in cultivars and media. The two-way interaction was also highly significant. The percentage of explants that developed calli ranged from 78.3% (‘Lia’) to 58.6% (‘Farida’). MS medium supplemented with 0.5 mg/l of NAA + 0.5 mg/l of BAP (Medium B) and medium supplemented with 0.01 mg/l of NAA + 1.0 mg/l of BAP (Medium C) gave the highest average of callus induction. The cultivar Banyas produced the highest callus weight with medium A, B and C on the other hand, the cultivar Miledy produced the highest callus weight with medium D. The highest percentage of embryogenic callus resulted from ‘Miledy’ cultivar. The percentage of root formation ranged from 0.0% (‘Miledy’) to 40.0% (‘White Liberty’). Results also showed that the cultivars Lia and White Liberty produced the highest mean values of shoot formation (15.5 and 13.8%, respectively) when calli differentiated on MS medium supplemented with 0.2 mg/l NAA and 1.0 mg/l BAP. 

Keywords:

Carnation, Dianthus caryophyllus L., in vitro culture, plant regeneration

---

Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 1
Category: Environment
Pages: 1113-1117

---

Full text for Subscribers

---

Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

---

Purchase this Article:   20 Purchase PDF Order Reprints for 15

---

Share this article :