Journal of Food, Agriculture and Environment

Vol 12, Issue 1,2014
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Biological assays of plant extracts from Araucaria columnaris and Cycas revoluta


Mehmood Zaffar 1, Muhammad Farooq Hussain Munis 1, Fahd Rasul 2, Sajid Masood 1, Syed Waqas Hassan 1, Muhammad Imran1, Wajid Nasim 3, Ali Zakir 1, Umar Masood Quraishi 1, Hassan Javed Chaudhary 1 *

Recieved Date: 2013-10-24, Accepted Date: 2014-01-10


In present investigation, organic extracts (methanol, ethanol and ethyl acetate) from leaves of Araucaria columnaris Hook and Cycas revoluta Thunb were subjected to the potential antibacterial and antioxidant activities. The extracts were evaluated for their effectiveness against six bacterial strains including both Gram-positive and Gram-negative bacteria using Agar well diffusion method. The antioxidant potential of extracts was determined on the basis of their scavenging activity of the stable 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical. It was evident that these extracts from both plants were active against the bacteria under observation at low concentrations. However, maximum zone of inhibition (40.33±0.66a mm) was produced by methanolic extract from Cycas revoluta. IC50 of the methanol extract of Cycas revoluta was 110.25 µg/ml, which indicated the strong antioxidant activity of the plant. The results obtained from preliminary analysis suggest the presence of active secondary metabolites and it is recommended that further intensive studies should be carried out to explore hidden potential.


Antibacterial, antioxidant, organic extracts, biological assays, medicinal plants, DPPH

Journal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 1
Category: Agriculture
Pages: 128-131

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