Journal of Food, Agriculture and Environment

Applications of salt and drought stress on the antioxidative enzyme activities and malondialdehyde content in callus tissues of pumpkin genotypes 

Author(s):

Sebnem Kusvuran ¹*, Sebnem Ellialtioglu ², Zehra Polat ²

Recieved Date: 2012-11-10, Accepted Date: 2013-04-26

Abstract:

The responses to salt and drought stress of 4 pumpkin varieties (A-24, CU-7, Iskenderun-4 and AB-44) were investigated under in vitro culture conditions. Of the 4 pumpkin varieties, 2 were salt-sensitive and the other 2 were salt-tolerant. Growth inhibition in callus tissues was observed in media that contained 100 mM NaCl or 15% (w/v) of polyethylene glycol (PEG-6000) compared to the control in all of the varieties on the 4^th and  8^th day of the salt and drought stress. As it is known, the malondialdehyde measurement can show the amount of lipid peroxidation in the cell wall. In this study, the magnitude of cell wall damage (lipid peroxidation) was lower in the tolerant genotypes than in the sensitive ones. On the other hand, the pumpkin genotypes were more affected by salt stress than by drought stress. The amount of lipid peroxidation was higher in the salt-stressed calli than in those that were drought-stressed. Besides the MDA measurements, the antioxidant enzyme (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase) activities were also investigated in the callus tissues of 4 pumpkin genotypes under salt and drought stress. The antioxidant enzyme activities showed differences among pumpkin genotypes. 

Keywords:

Drought, salinity, CAT, APX, SOD

---

Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 2
Category: Agriculture
Pages: 496-500

---

Full text for Subscribers

---

Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

---

Purchase this Article:   20 Purchase PDF Order Reprints for 15

---

Share this article :