Journal of Food, Agriculture and Environment

Vol 11, Issue 3&4,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Heat exposure upregulates expression of cytokines and aggravates lung injury in Klebsiella pneumoniae – Challenged rats


Jiansheng Li 1, 2*, Xue Mei 1, Hongyan Zhou 1, Yanxia Zhang 1, Suyun Li 2

Recieved Date: 2013-06-14, Accepted Date: 2013-10-29


To demonstrate impact of thermal environment on klebsiellar pneumonia and the underlying molecular mechanism, Wistar rats were randomly divided into four groups: 1) no-treatment control (n = 12), 2) heat-treatment alone (n = 12), 3) K. pneumoniae pneumonia alone (n = 12), and 4) K. pneumoniae pneumonia plus heat treatment (n = 12). White blood cells (WBC) and polymorphonuclear neutrophils (PMN) percentage in peripheral blood were examined. Pathological changes of lung tissue were observed. IL-6, TNF-αand ICAM-1 in the bronchoalveolar lavage fluid were evaluated by radioimmunoassay. IL-1β, TNF-αand IL-6 protein expressions in lung tissue were analyzed by immunohistochemistry staining. The expression of IL-1βmRNA was measured by RT-PCR. Histopathological study indicated that there was pronounced damage in the heat + pneumonia group. Significant elevation of TNF-α, IL-6 and levels in the bronchoalveolar lavage fluid and lung tissue was observed in the heat + pneumonia group compared to the heat-alone group or the pneumonia-alone group. ICAM-1 level in the bronchoalveolar lavage fluid was higher in the heat+ pneumonia group compared to the heat-alone group. However, there was no difference in IL-1βlevel between the heat + pneumonia group and the pneumonia-alone group. The main findings of our study were that heat exposure increased levels of inflammation cytokines and aggravated pathological injury of lung tissue in Klebsiella pneumoniae pneumonia rats.


Klebsiella pneumoniae pneumonia, interleukin -1β, Interleukin-6, tumor necrosis factor-α, intercellular adhesion molecule-1, lung injury

Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Food and Health
Pages: 139-144

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