Journal of Food, Agriculture and Environment

Production of cordycepin by Cordyceps militaris using submerged liquid culture: Optimization of the culture medium and repeated batch fermentation

Author(s):

Jian-Guo Zhang #, Ting-Ting Fang #, Qiao-Liang Li, Zhao-Jun Wei *

Recieved Date: 2013-07-03, Accepted Date: 2013-10-20

Abstract:

Cordycepin (3’-deoxyadenosine) is one of the most versatile metabolites of Cordyceps militaris due to its broad spectrum of biological activity. In this study, response surface methodology (RSM) was applied to optimize the medium components for the cordycepin production by submerged liquid culture. The optimal parameters for cordycepin production within the experimental range of the variables researched was at 30.16 g/L glucose, 10.00 g/L yeast extract, 2.040 g/L KH₂PO₄, and 1.460 g/L MgSO₄. At this condition, the cordycepin was predicted to be 63.69 mg/L. The maximum cordycepin obtained experimentally was 64.15 mg/L, which confirmed the model prediction. The repeated batch operation was proved to be an efficient method to increase the cordycepin yield. During the repeated batch fermentation, the cordycepin yields and productivity of the 2^nd to 6^th cycles are higher than that of 1^st cycle.

Keywords:

Cordyceps militaris, cordycepin, medium component, response surface design, repeated batch operation

---

Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Food and Health
Pages: 534-538

---

Full text for Subscribers

---

Information:

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

---

Purchase this Article:   20 Purchase PDF Order Reprints for 15

---

Share this article :