Journal of Food, Agriculture and Environment

Vol 11, Issue 3&4,2013
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Production of cordycepin by Cordyceps militaris using submerged liquid culture: Optimization of the culture medium and repeated batch fermentation


Jian-Guo Zhang #, Ting-Ting Fang #, Qiao-Liang Li, Zhao-Jun Wei *

Recieved Date: 2013-07-03, Accepted Date: 2013-10-20


Cordycepin (3’-deoxyadenosine) is one of the most versatile metabolites of Cordyceps militaris due to its broad spectrum of biological activity. In this study, response surface methodology (RSM) was applied to optimize the medium components for the cordycepin production by submerged liquid culture. The optimal parameters for cordycepin production within the experimental range of the variables researched was at 30.16 g/L glucose, 10.00 g/L yeast extract, 2.040 g/L KH2PO4, and 1.460 g/L MgSO4. At this condition, the cordycepin was predicted to be 63.69 mg/L. The maximum cordycepin obtained experimentally was 64.15 mg/L, which confirmed the model prediction. The repeated batch operation was proved to be an efficient method to increase the cordycepin yield. During the repeated batch fermentation, the cordycepin yields and productivity of the 2nd to 6th cycles are higher than that of 1st cycle.


Cordyceps militaris, cordycepin, medium component, response surface design, repeated batch operation

Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Food and Health
Pages: 534-538

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