Journal of Food, Agriculture and Environment

Regulation of type II sodium-dependent phosphate transporters in caprine parotid gland, small intestine and kidney by dietary phosphorus and protein levels

Author(s):

Qiongxian Yan ^{1, 2}, Shaoxun Tang ^1a, Zhiliang Tan ¹*, Xuefeng Han ¹, Chuanshe Zhou ¹, Jinhe Kang ¹, Min Wang ¹

Recieved Date: 2013-07-10, Accepted Date: 2013-10-19

Abstract:

Relationships between dietary crude protein (CP) and inorganic phosphorus (Pi) intake and Pi metabolism are inconsistent among ruminant species. The aim of this study was to investigate whether this relates to the difference in expression of type II Na⁺-dependent Pi transporters (NaPi II) in different tissues. Sixteen adult goats were randomly allotted to four experimental diets consisted of two Pi levels (0.159% and 0.194%, DM basis) and CP levels (9.2% and 12.0%, DM basis) within a 2 × 2 factorial design. The relative amounts of NaPi II mRNA and protein were determined in the parotid gland, small intestine and kidney. The results showed that the expression of NaPi II b mRNA and protein in the parotid gland was not affected by dietary Pi or CP supply. The relative abundance of NaPi II b mRNA was up-regulated (P < 0.05) in the ileum for goats fed low CP diet. Renal NaPi II a mRNA was stimulated (P < 0.05) by low Pi diet under low CP; while the protein expression was increased (P < 0.05) by high dietary CP level. In addition, there was an interaction (P < 0.05) between dietary CP and Pi levels on the expression of renal NaPi II a mRNA. These results indicate that ileum Na⁺-dependent Pi transport systems respond to dietary Pi and CP levels via regulation of NaPi II b mRNA; while the regulation in the kidney is through expression of NaPi II a transporter at both the mRNA and protein levels.

Keywords:

Goats, crude protein, inorganic phosphorus, NaPi II a, NaPi II b

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Agriculture
Pages: 924-930

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